The Comet assay (single cell gel electrophoresis) is a sensitive technique for the detection of single- and double-strand breaks and alkaline-labile sites in nuclear DNA of individual cells. The Comet assay has the added advantage of detecting apoptotic/necrotic cells, which can be clearly distinguished from viable cells exhibiting DNA strand breaks.
Our targets through this blog are,
1.
Integrating the knowledge of comet assay.
2. Documentation of ongoing research work based on comet assay conducted by us.
3. Sharing the knowledge & experience gained through our research.
4. Convenient easy access to information on the procedure and the results.
5. Free accessibility to information comparative to other sources.
6. To enhance awareness & talent on subject & the updation of new development of assay.

Monday, March 22, 2010

References for comet asaay

Collins AR. Comet Assay for DNA damage and repair: principles, applications and limitations. Mol. Biotechnol. 2004; 26: 249-61.

Dixon DR, Pruski AM, Dixon LRJ, Jha AN. Marine invertebrate eco-genotoxicology: a methodological overview. Mutagenesis. 2002; 17: 495-507.

Fairbairn DW, Olive PL, O’Neill KL. The Comet Assay: A comprehensive review. Mutat. Res. 1995; 339: 37-59.

Fisher AE, Burke D, Routledge MN. Can irradiation of rectal tumour cells from patient biopsy predict outcome of radiotherapy? Proceedings of the Genome Stability network/United Kingdom Environmental Mutagen Society Joint Congress, University of Cardiff, 1 – 4 July 2007.

Gichner T, Mukherjee A, Veleminsky J. DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I. Mutat Res. 2006; 605: 17-21.

Gichner T, Patkova Z, Szakova J, Demnerova K. Cadmium induces DNA damage in tobacco roots, but no DNA damage, somatic mutations or homologous recombination in tobacco leaves. Mutat Res. 2004; 559: 49-57.

Collins AR, Dusinská M, Horská A. Detection of alkylation damage in human lymphocyte DNA with the comet assay. Acta Biochim. Pol. 2001; 48: 611–14

Bichler J, Cavin C, Simic T, Chakraborty A, Ferk F, Hoelzl C, Schulte-Hermann R, Kundi M, Haidinger G, Angelis K, Knasmüller S. Coffee consumption protects human lymphocytes against oxidative and 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetate (Trp-P-2) induced DNA-damage: Results of an experimental study with human volunteers. Food Chem. Toxicol. 2007 Epub ahead of print.

Burlinson B, Tice RR, Speit G, Agurell E, Brendler-Schwaab SY, Collins AR, Escobar P, Honma M, Kumaravel TS, Nakajima M, Sasaki YF, Thybaud V, Uno Y, Vasquez M, Hartmann A. In Vivo Comet Assay Workgroup, part of the Fourth International Workgroup on Genotoxicity Testing: results of the in vivo Comet Assay workgroup. Mutat. Res. 2007; 627: 31-5.

Collins AR, Duthie, SJ, Dobson VL. Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis. 1993; 14: 1733–735.Collins A, Dusinska M, Franklin M, Somorovska M, Petrovska H, Duthie S, Fillion L, Panayiotidis M, Raslova K, Vaughan N. Comet Assay in human biomonitoring studies: reliability, validation, and applications. Environ. Mol. Mutagen. 1997; 30: 139–46.

Bichler J, Cavin C, Simic T, Chakraborty A, Ferk F, Hoelzl C, Schulte-Hermann R, Kundi M, Haidinger G, Angelis K, Knasmüller S. Coffee consumption protects human lymphocytes against oxidative and 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetate (Trp-P-2) induced DNA-damage: Results of an experimental study with human volunteers. Food Chem. Toxicol. 2007 Epub ahead of print.

Burlinson B, Tice RR, Speit G, Agurell E, Brendler-Schwaab SY, Collins AR, Escobar P, Honma M, Kumaravel TS, Nakajima M, Sasaki YF, Thybaud V, Uno Y, Vasquez M, Hartmann A. In Vivo Comet Assay Workgroup, part of the Fourth International Workgroup on Genotoxicity Testing: results of the in vivo Comet Assay workgroup. Mutat. Res. 2007; 627: 31-5.

Collins AR, Duthie, SJ, Dobson VL. Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis. 1993; 14: 1733–735.Collins A, Dusinska M, Franklin M, Somorovska M, Petrovska H, Duthie S, Fillion L, Panayiotidis M, Raslova K, Vaughan N. Comet Assay in human biomonitoring studies: reliability, validation, and applications. Environ. Mol. Mutagen. 1997; 30: 139–46.

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